K. Koch,
F. Zöllner, S. Neumann, F. Kummert, and G. Sagerer:
Comparing bound and unbound
protein structures
using energy calculation and rotamer statistics.
In Silico Biology, 2:32, 2002.
Protein data in the PDB covers only a snapshot of a protein
structure. For flexible docking conformational changes need
to be considered. Rotamer statistics provide the likelihood
for side chain conformations, and further comparison of bound
and unbound state yields differences in preferred positions.
Furthermore, we do a full sampling of selected Chi angles and
apply the AMBER force field. Conformation of energy minima comply
with the rotamer statistics. Both types of information target
the reduction of search space for enumerative docking algorithms
and provide parameters for elastic docking.
Key words: Rotamer library, flexible
protein-protein docking, energy calculations, AMBER force field,
side chain flexibility, flexibility measure.