M. Katzer, F. Kummert, and G. Sagerer:

Methods for Automatic Microarray Image Segmentation.

IEEE Transactions on Nano-Bioscience, 2(4):202--214, 2003.
This article describes image processing methods for automatic spotted microarray image analysis. Automatic gridding is important to achieve constant data quality and is therefore especially interesting for large scale experiments as well as for integration of microarray expression data from different sources. We propose a Markov random field based approach to high level grid segmentation, which is robust to common problems encountered with array images and does not require calibration. We also propose an active contour method for single spot segmentation. Active contour models describe objects in images by properties of their boundaries. Both Markov random fields and active contour models have been used in various other computer vision applications. The traditional active contour model must be generalized for successful application to microarray spot segmentation. Our active contour model is employed for spot detection in the MRF score functions as well as for spot signal segmentation in quantitative array image analysis. An evaluation using several image series from different sources shows the robustness of our methods.