Mitarbeiter am Lehrstuhl für Fermentationstechnik

Dr. Carsten Voß

Dr. Carsten Voß,
Assistant Professor



Diese Seite auf deutsch.
Tel +49 (0)5 21 - 106-5287 (office)
Tel +49 (0)5 21 - 106-5490 (lab)
Email: cvo@fermtech.techfak.uni-bielefeld.de
Curriculum vitae | Research | Contact | Teaching | References
Curriculum vitae

April 2001 Appointment as Assistant Professor (Wissenschaftlicher Assistent) at the Chair of Fermentation Engineering, Faculty of Technology, Bielefeld University, Germany.
1997-2001 Doctoral thesis. Title: "Neue Verfahren zur Produktion von Plasmid-DNA als Biopharmazeutikum (Novel processes for biopharmaceutical plasmid DNA production".
1996-1997 Diploma thesis at the chair of Fermentation Engineering, Faculty of Technology, Bielefeld University, Germany. Title: "Methoden zur Quantifizierung von DNA: Vergleich und Optimierung (DNA quantitation methods: comparison and optimization)."
1991-1996 Studies in chemistry at the faculty of chemistry, Bielefeld University, Germany
Research

Main focus of our research is put on fermentation engineering, downstream processing of biotechnological products, and development of analytical methods for process- and product quality-control.

The development of high cell density cultivation processes especially for pharmaceutical plasmid DNA production is a major topic in our research in fermentation engineering.

In the area of downstream processing the development of product isolation processes for large scale biotechnological production is a major goal. Since plasmid DNA is gaining more and more importance as a pharmaceutical drug, its production is of special interest. Areas of our research on this topic are the scale-up of cell-disruption methods and further downstream-processing steps, the extraction of nucleic acids, and the development of nucleic acid specific affinity ligands based on protein-DNA interaction.

Technologies for process and product quality-control are developed using capillary electrophoresis systems. Methods for plasmid quantitation and analysis of plasmid homogeneity by capillary gel electrophoresis (CGE) have already been developed at our chair.

Contact

If you are interested in one of the topics mentioned above (production of biomass for plasmid DNA manufacturing, downstream processing, analytical methods, CE, CGE) you can contact Dr. Carsten Voß using the adress given below.
Students and trainees interested in practical training in our area can also make direct contact to Dr. Voß.

Dr. Carsten Voß
Chair of Fermentation Engineering
Faculty of Technology
University of Bielefeld
Universitätsstraße 25
D-33615 Bielefeld
Germany

Tel +49 (0)5 21 - 106-5287 (office)
Tel +49 (0)5 21 - 106-5490 (lab)
Fax +49 (0)5 21 - 106-6475
E-mail: cvo@fermtech.techfak.uni-bielefeld.de

Teaching

Senior students in molecular biotechnology have the opportunity to make a project in one of our research areas. Interested persons can contact Dr. Voß (D5-124 or D5-Laboratory).
Students in biochemistry and chemistry also have the opportunity to start a blockwise practical training (Blockpraktikum) in one of research areas.

In our molecular biotechnology course the specialisation "Downstream Processing of Biotechnological Products" is offered. It contains lecture (2 SWS), seminar (1 SWS), and a practical training (6 SWS). Specialization is taking place during winter- and summersemester.

Dr. Voß is also conducting practical training "Basic Biotechnology II" and "Biotechnology for Biochemistry Students".

References

Paper

Schmidt, T., Friehs, K., Schleef, M., Voß, C., Flaschel, E. (1999), Quantitative analysis of plasmid forms by agarose and capillary electrophoresis. Anal. Biochem. 274, 235-240.

Schmidt, T., Friehs, K., Schleef, M., Voß, C., Flaschel, E. (2000), Assessing the homogeneity of plasmid DNA: an important step toward gene-therapy. PACE Setter 4, 1-3.

Schmidt, T., Schleef, M., Voß, C., Flaschel, E. (2000), CGE-Analyse von Plasmid-DNA für klinische und veterinär-medizinische Applikationen. BIOforum 12, 877-878.

Walther, W., Stein, U., Fichtner, I, Voß, C., Schmidt, T., Schleef, M., Nellessen, T., Schlag, P.M. (2002), Intratumoral low volume jet-injection for efficient nonviral gene transfer. Mol. Biotechnol. 21, 105-115.

Schleef, M., Voß, C., Schmidt, T. (2002), Novel technology for topological plasmid vaccine characterization. Nature Labscene 4,1-2.

Schleef, M., Voß, C., Schmidt, T. (2002), DNA drugs - production and quality assurance. Eng. Life Sci. 2, 157-160.

Schleef, M., Schmidt, T., Voß, C. (2002), Upgrade plasmid vectors - the topology as an important factor. Biotechnology International 14, 19.

Voß, C., Schmidt, T., Schleef, M., Friehs, K., Flaschel, E. (2003), Production of supercoiled multimeric plasmid DNA for biopharmaceutical application. J. Biotechnol. 105, 205-213.

Walther, W., Stein, U., Voß, C., Schmidt, T., Schleef, M., Schlag, P.M. (2003). Stability analysis for long-term storage of naked DNA: impact on non-viral in vivo gene transfer. Anal. Biochem. 318, 230-235.

Voss, C., Schmidt, T., Schleef, M., Friehs, K., Flaschel, E. (2004), Effect of ammonium chloride on plasmid DNA production in high cell density batch culture for biopharmaceutical use. J. Chem. Technol. Biotechnol. 79, 57-62.

Patente und Offenlegungsschriften

Voß, C. (2002). Method for the production of nucleic acids. WO02064752.

Friehs, K., Flaschel, E., Schleef, M., Schmidt, T., Voß, C. (2002). Method for producing homogeneous nucleic acid multimers for use in pharmaceutical compositions. WO02062987.

Konferenzbeiträge

Voß, C., Schmidt, T., Schleef, M., Friehs, K., Flaschel, E. (1998), Vergleich von Methoden zur Quantifizierung von DNA. 16. Jahrestagung der Biotechnologen, Wiesbaden.

Voß, C., Schmidt, T., Schleef, M., Friehs, K., Flaschel, E. (1999), Qualitätssicherung von Plasmid-DNA für die somatische Gentherapie und die genetische Vakzinierung. 17. Jahrestagung der Biotechnologen, Wiesbaden.

Voß, C., Schmidt, T., Schleef, M., Friehs, K., Flaschel, E. (2000), Biomass production for the isolation of plasmid DNA for genetic vaccination and gene therapy. Biotechnology 2000, Berlin.

Schleef, M., Schmidt, T., Voß, C., Flaschel, E. (2001), Novel quality assurance testing for plasmid DNA used in gene therapy or human and veterinary vaccination. 19. Jahrestagung der Biotechnologen, Leipzig.

Schleef, M., Voß, C., Schmidt, T., Flaschel, E. (2001), Plasmid topology - an important factor influencing the performance of plasmid vectors. GVPN Conference, Oct. 4-5 2001, Evry, France

Voß, C., Schmidt, T., Schleef, M. , Flaschel, E. (2001), Biomass production for pharmaceutical plasmid-DNA manufacturing. GVPN Conference, Oct 4-5 2001, Evry, France.

Voß, C., Schmidt, T., Stadler, J., Schleef, M., Flaschel, E. (2001), Innovative in-process control and quality control in supercoiled plasmid DNA production. 9th ESGT Meeting, Nov. 2-4 2001, Antalya, Turkey.

Schleef, M., Voß, C., Schmidt, T. (2002), Analytical and preparative separation of plasmid topologies in DNA vaccines. DNA Vaccine meeting 2002.

Voß, C., Schmidt, T., Schleef, M., Flaschel, E. (2002). Produktion von Plasmid-DNA für pharmazeutische Anwendungen. 20. Jahrestagung der Biotechnologen, Wiesbaden.

Schulze-Horsel, J., Voß, C., Flaschel, E. (2003). Kultivierung von Escherichia coli unter anaeroben Bedingungen. 21. Jahrestagung der Biotechnologen, Garching.

Voß, C., Schmidt, T., Schleef, M., Flaschel, E. (2003). Herstellung von Plasmid-Multimeren als Biopharmazeutikum. 21. Jahrestagung der Biotechnologen, Garching.

Voß,C. (2004). Downstream processing strategies for plasmid DNA as a pharmaceutical drug. (Lecture). 22. Jahrestagung der Biotechnologen/Bioperspectives 2004, Wiesbaden.